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Microbial responses to salt-induced osmotic stress
Authors:D R Polonenko  C I Mayfield  E B Dumbroff
Institution:(1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada;(2) Present address: Allelix Inc., 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada
Abstract:Summary The construction and assembly of a model root region is described. The model was used to manipulate the soil matrix, soil microorganisms, and to simulate release of root exudates. The design of the apparatus facilitated long-term, direct microscopic observations of microbial activity in soil and on artificial roots. Preliminary studies indicate that microbial responses to osmotic stress and to changes in components of exudate solutions are easily monitored.
Keywords:Fluorescence microscopy  Exudates  Microbial ecology  Model root  Rhizosphere
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