Glycolipid modulation of membrane adhesion |
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Authors: | Gregory J. Brewer P. D. Thomas |
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Affiliation: | (1) Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, 62708 Springfield, Illinois |
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Abstract: | A novel test of carbohydrate-mediated adhesion has been developed. At the tips of two syringes, large spherical model membranes have been made from phosphatidylcholine and varying amounts of mixed brain gangliosides dissolved inn-decane. The apposition of two such membranes resulted in adhesion, not fusion, as judged by the absence of fluorescence mixing in the junction with NBD-phosphatidylethanolamine in one membrane and perylene in the other. Adhesion was observed without gangliosides. The rate of formation of the adhesion area (rate of adhesion) was unchanged from 0 to 0.8 mol% gangliosides. A slightly lower but constant rate was observed within the physiological range from 2 to 10 mol%. Adhesion was frequently blocked at 11 to 15 mol% gangliosides. The rate of adhesion with pure gangliosides increased with the number of sialic acid residues: GT1>GD1a>GM1. These results are interpreted in terms of a sialic acid-dependent segregation of gangliosides into the adhesion zone. |
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