| 穿山龙多糖的提取、纯化与抗氧化活性研究 |
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| 引用本文： | 王昭晶,罗巅辉.穿山龙多糖的提取、纯化与抗氧化活性研究[J].天然产物研究与开发,2007,19(1):29-34. |
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| 作者姓名： | 王昭晶 罗巅辉 |
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| 作者单位： | 华侨大学生物工程与技术系,泉州,362021 |
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| 摘 要： | 采用热水煮提法和乙醇沉淀法从穿山龙中提取出水溶性粗多糖,再经DEAE-Sepharose CL-6B和Sepha-dex G-100柱层析分离纯化得到2个均多糖组分DMA和DMB。利用超氧阴离子实验,羟基阴离子实验和邻苯三酚自氧化实验研究DMA和DMB的抗氧化活性,结果表明DMB有较强的抗氧化活性,应该被作为潜在的抗氧化剂开发利用。
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| 关 键 词： | 穿山龙 多糖 纯化 抗氧化活性 |
| 文章编号： | 1001-6880（2007）01-0029-06 |
| 收稿时间： | 2006-06-28 |
| 修稿时间： | 2006-11-07 |
Extraction, Purification and Antioxidant Activities of Polysaccharide from Dioscorea nipponica Makino |
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| WANG Zhao-jing,LUO Dian-hui.Extraction, Purification and Antioxidant Activities of Polysaccharide from Dioscorea nipponica Makino[J].Natural Product Research and Development,2007,19(1):29-34. |
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| Authors: | WANG Zhao-jing LUO Dian-hui |
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| Institution: | Department of Bioengineering and Biotechnology ,Huaqiao University, Quanzhou 362021, China |
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| Abstract: | The water-soluble crude polysaccharide,obtained from the Dioscorea nipponica Makino by boiling-water extraction and ethanol precipitation,was fractionated by DEAE-Sepharose CL-6B column chromatography and purified by Sephadex G-100,giving two polysaccharide fractions termed DMA and DMB.On the basis of superoxide radical assay,hydroxyl radical assay and self-oxidation of 1,2,3-phentriol assay,their antioxidant activities were investigated.Among them,DMB had the higher antioxidant activities and should be explored as a novel potential antioxidant agent. |
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| Keywords: | Dioscorea nipponica Makino polysaccharide purification antioxidant activities |
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