鄂尔多斯台地盐沼滩涂湿地土壤细菌群落结构及特征

依据植被分类法将鄂尔多斯高原盐沼滩涂湿地划分为肉质耐盐草甸(B)、苔草草甸(C)、禾草草甸(D)和杂类草草甸(E)等4个植被亚型，并以盐沼裸地(A)为对照样地，共计5种盐沼滩涂湿地景观类型。运用高通量测序技术分别研究其土壤细菌群落结构特征、分布情况，以及土壤盐分与土壤细菌之间的关系。结果表明:(1) 5种盐沼滩涂湿地的土壤细菌样品共12213条OTUs，属于45个门，122个纲，365个目，663个科，1375个属，2882个种。(2)变形菌门(26.19%)是盐沼滩涂湿地平均相对丰度最高的门，其次为放线菌门(17.15%)，绿弯菌门(12.62%)，芽单胞菌门(Gemmatimonadetes,11.23%)，拟杆菌门(Bacteroidetes,9.38%)，酸杆菌门(Acidobacteria,8.83%)厚壁菌门(Firmicutes,2.96%);芽单胞菌纲中的未定细菌(norank＿c＿Gemmatimonadetes)是丰度最高的属，平均丰度为5.75%。(3)鄂尔多斯盐沼滩涂湿地自西南向东北，空间位置相近的土壤细菌群落结构具有更大的相似性，随着土壤盐分变化，变形菌门相对...

Characteristics and distribution of soil bacterial of salt marsh tidal wetland in Ordos Platform

According to the vegetation classification method, the salt marsh tidal wetland in Ordos Plateau was divided into 4 vegetation subtypes, such as succulent salt-tolerant meadow (B), carex meadow (C), grass meadow (D) and forbs meadow (E). meanwhile, salt marsh bare land (A) was taken as the control plot, there are five landscape types of salt marsh tidal wetland designed. The structure characteristics and distribution of soil bacterial community and the relationship between soil salinity and soil bacteria were studied by high-throughput sequencing technique. The results showed that:(1) There were 12213 OTUs in the soil bacteria samples of 5 kinds of salt marsh tidal wetland, which belonged to 2882 species, 1375 genera, 663 families, 365 orders, 122 classes and 45 phyla. (2) Proteobacteria (26.19%) was the highest average relative abundance of salt marsh tidal wetland, followed by Actinobacteria (17.15%), Chloroflexi (12.62%), Gemmatimonadetes (11.23%), Bacteroidetes (9.38%), Acidobacteria (8.83%) and Firmicutes (2.96%); the undetermined bacterium (norank_c_Gemmatimonadetes) in Gemmatimonadetes was the most abundant genus with an average abundance of 5.75%. (3) The community structure of soil bacteria with close spatial positions had a greater similarity from southwest to northeast in Ordos salt marsh tidal wetland. With the change of salt content in soil, the relative abundance of Proteobacteria increased at first and then decreased, which was contrary to the change trend of Deinococcus-Thermus; the relative abundance of Chloroflexi and Acidobacteria showed an increasing trend. Bacteroidetes and Firmicutes generally showed a decreasing trend, and Gemmatimonadetes and Rokubacteria showed a fluctuating and unstable change trend. (4) The abundance and diversity of soil bacterial community in different salt marsh tidal wetlands were as follows:carex meadow > forbs meadow > grass meadow > succulent salt-tolerant meadow > salt marsh bare land. (5) The correlation between soil salinity and bacterial community in Ordos salt marsh tidal wetland from high to low was as follows:TS > CO₃^2-> Na ⁺ > Cl^-> pH > SO₄^2-> K ⁺ > Mg^{2 +} > Ca²⁺> HCO₃^-. The results of this study could not only provide theoretical basis for revealing the composition of soil bacterial flora in salt marsh tidal wetland of Ordos Plateau and the change law of its corresponding environmental characteristics, but also further explored the relationship between soil salinity and soil microorganisms, so as to provide reference for soil remediation and habitat succession in salt marsh areas of Ordos Plateau.