Preparation techniques for the electron microscopy of granular sludge from an anaerobic digester |
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| Authors: | A.R. Howgrave-Graham F.M. Wallis |
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| Affiliation: | Department of Microbiology and Plant Pathology, University of Natal, PO Box 375, Pietermaritzburg, 3200, South Africa |
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| Abstract: | Several fixation and dehydration techniques for scanning and transmission electron microscopy of glycocalyx and microbial populations within granules from an upflow anaerobic sludge blanket digester purifying a brewery wastewater were compared. Sputter-cryo and freeze-drying techniques prior to scanning electron microscopy (SEM) allowed viewing of the glycocalyx. In contrast standard fixation and dehydration techniques were suitable for examination of underlying microbial populations by both SEM and transmission electron microscopy. |
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