新疆阿克苏地区盐碱地细菌类群多样性及优势菌群分析

【目的】研究新疆阿克苏地区盐碱土样中细菌类群多样性和优势种群,及其与环境因子的相关性。【方法】采用基于16S rDNA的变性梯度凝胶电泳(Denaturing gradient gel electrophoresis,DGGE)、克隆测序和典型相关性分析(CCA)的方法。【结果】优势菌群序列分析表明有29个序列属于未培养的微生物,其他43个序列分属细菌的9个目:粘球菌目(Myxococcales)、假单胞菌目(Pseudomonadales)、根瘤菌目(Rhizobiales)、芽孢杆菌目(Bacillales)、伯克氏菌目(Burkholderiales)、放线菌目(Actinomycetales)、海洋螺菌目(Oceanospirillales)、黄杆菌目(Flavobacteriales)、交替单胞菌目(Alteromonadales),21个属。【结论】新疆阿克苏地区土壤中的微生物丰富度非常高,存在大量的细菌类群,优势菌群不尽相同,盐碱土样中微生物群落结构与环境因子是密切相关的。

Analysis of the bacterial diversity and dominant population in Akesu Saline-alkali in Xinjiang

Objective] The research was conducted to study on the bacterial community diversity and dominant population in Xinjiang akesu area and the correlation with the environmental factor. Methods] The bacteria diversity and dominant population was analysis by PCR-denaturing gradient gel electrophoresis (DGGE), cloning, sequencing and the DGGE sequence analysis and the canonical correlation analysis (CCA). Results] The sequencing of the dominant population showed that there were 29 sequencing belong to the un-cultivation microbial, and the other 43 DGGE band were made up by 9 order: Myxococcales, Pseudomonadales, Rhizobiales, Bacillales, Burkholderiales, Actinomycetales, Oceanospirillales, Flavobacteriale, Alteromonadales. Conclusion] Canonical correlation analysis (CCA) result showed that microbial community structure and environmental factors were closely related in saline-alkaline soils.