Killing activity of LFchimera on periodontopathic bacteria and multispecies oral biofilm formation in vitro |
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Authors: | Sopita Ruangcharoen Waraporn Suwannarong Marie Rossini Carmela T. Lachica Jan G. M. Bolscher Kamran Nazmi Watcharee Khunkitti Suwimol Taweechaisupapong |
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Affiliation: | 1.Biofilm Research Group, Faculty of Dentistry,Khon Kaen University,Khon Kaen,Thailand;2.Department of Periodontology, Faculty of Dentistry,Khon Kaen University,Khon Kaen,Thailand;3.Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA),University of Amsterdam and VU University Amsterdam,Amsterdam,The Netherlands;4.Faculty of Pharmaceutical Sciences,Khon Kaen University,Khon Kaen,Thailand;5.Department of Oral Diagnosis, Faculty of Dentistry,Khon Kaen University,Khon Kaen,Thailand |
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Abstract: | Lactoferrin chimera (LFchimera), a heterodimeric peptide containing lactoferrampin (LFampin265–284) and a part of lactoferricin (LFcin17–30), possesses a broad spectrum of antimicrobial activity. However, there is no report on the inhibitory effects of LFchimera against multispecies oral biofilms. This study aimed to determine the effects of LFchimera in comparison to chlorhexidine digluconate (CHX) and minocycline hydrochloride (MH), on in vitro multispecies biofilms derived from subgingival plaque of periodontitis patients harboring Aggregatibacter actinomycetemcomitans. First the effects of LFchimera against planktonic and an 1-day old biofilm of the periodontopathic bacteria, A. actinomycetemcomitans ATCC 43718 were established. Then, the effects on biofilm formation and bacterial viability in the multispecies biofilm were determined by crystal violet staining and LIVE/DEAD BacLight Bacterial Viability kit, respectively. The results revealed that a significant reduction (P?0.05) in biofilm formation occurred after 15 min exposure to 20 µM of LFchimera or CHX compared to control. In contrast, MH at concentration up to 100 µM did not inhibit biofilm formation. The ratio of live/dead bacteria in biofilm was also significantly lower after 15 min exposure to 20 µM of LFchimera compared to control and 20–50 µM of CHX and MH. Altogether, the results obtained indicate that LFchimera is able to inhibit in vitro subgingival biofilm formation and reduce viability of multispecies bacteria in biofilm better than CHX and MH. |
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