Activation of a calcium-dependent protein kinase involved in the <Emphasis Type="Italic">Azospirillum</Emphasis> growth promotion in rice |
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Authors: | Claudia M Ribaudo José A Curá María L Cantore |
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Institution: | 1.Cátedra de Bioquímica, Facultad de Agronomía,Universidad de Buenos Aires,Ciudad Autónoma de Buenos Aires,Argentina |
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Abstract: | Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2+-dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western-blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family. The fact that the amount of the protein did not change upon inoculation seems to indicate that a posttranslational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation. To our knowledge, this is the first report on the possible involvement of a Ca2+-dependent protein kinase in promotion of rice plants growth by A. brasilense. |
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