琼脂糖凝胶的合适浓度对于回收酶切后质粒载体的重要性

目的：探讨琼脂糖凝胶的不同浓度对回收不同大小的酶切后质粒载体纯度的影响。方法：将2种质粒载体酶切，并经不同浓度的琼脂糖凝胶电泳分析，通过比较酶切前和酶切后载体的相对位置，研究胶浓度对回收酶切后载体纯度的影响。结果：不同浓度的琼脂糖凝胶中，酶切前和酶切后载体的相对位置会发生变化，对能否成功回收到纯度高的酶切后DNA片段有重要影响；质粒大小不同，胶浓度的影响也不同。结论：合适的胶浓度对于回收酶切后质粒载体具有重要意义，应选择合适的胶浓度回收酶切后质粒载体。

The Significance of Suitable Concentration of Agarose Gel for Recovery of Digested Plasmid Vector

Objective： To study the effect of different concentrations of agarose gel on recovery of digested plasmid vectors with different length. Methods： Two plasmids with different length were digested with two restriction enzymes and analyzed by electrophoresis of agarose gel with different concentrations. By comparing the relative position of plasmids before and after digestion, the effect of different concentrations of agarose gel on recovery of digested plasmid vectors was studied. Results： The relative position of plasmids before and after digestion changed in agarose gel with different concentrations, which were important for recovery of high purity of digested DNA fragments. The concentrations of agarose gel have different effects on plasmids with different length. Conclusion： Suitable concentration of agarose gel is important for recovery of digested plasmid vector.