Interaction Between Syntaxin 8 and HECTd3, a HECT Domain Ligase |
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Authors: | Lisheng Zhang Liang Kang William Bond Nian Zhang |
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Institution: | (1) Laboratory of Mammalian Developmental Genetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA;(2) Department of Biology and Biochemistry, Bath University, Bath, UK;(3) Stower Institute for Medical Research, Kansas City, MO 64110, USA |
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Abstract: | Ubiquitination of proteins and their degradation within the proteasome has emerged as the major proteolytic mechanism used
by mammalian cells to regulate cytosolic and nuclear protein levels. Substrate ubiquitylation is mediated by ubiquitin (Ub)
ligases, also called E3 Ub ligases. HECT-E3 Ub ligases are characterized by the presence of a C-terminal HECT domain that
contains the active site for Ub transfer onto substrates. Among the many E3 Ub ligases, the family homologous to E6-Ap C-terminus
(HECT) E3 Ub ligases, which includes the yeast protein Rsp5p and the mammalian homolog NEDD4, AIP4/Itch, and Smurf, has been
shown to ubiquitylate membrane proteins and, in some instances, to induce their degradation. In this report, we have identified
Syntaxin 8 as a binding protein to a novel HECT domain protein, HECT domain containing 3 (HECTd3), by yeast two-hybrid screen.
Besides HECT domain, HECTd3 contains an anaphase-promoting complex, subunit 10 (APC10) domain. Our co-immunoprecipitation
experiments show that Syntaxin 8 directly interacts with HECTd3 and that the overexpression of HECTd3 promotes the ubiquitination
of Syntaxin 8. Immunofluorescence results show that Syntaxin 8 and HECTd3 have similar subcellular localization. |
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Keywords: | Syntaxin 8 Ubiquitination HECT E3 ubiquitin ligases HECTd3 |
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