Neuronal cell differentiation of mesenchymal stem cells originating from canine amniotic fluid |
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Authors: | Eun Young Kim Kyung-Bon Lee Jung Yu Ji Hye Lee Keun Jung Kim Kil-Woo Han Kang-Sun Park Dong-Soo Lee Min Kyu Kim |
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Affiliation: | 1. Laboratory of Animal Reproduction and Physiology, Department of Animal Science and Biotechnology, College of Agriculture Life Science, Chungnam National University, Daejeon, 305-764, Republic of Korea 2. Department of Regeneration and Advanced Medical Sciences, Graduate School of Medicine, Gifu University, Gifu, 501-1193, Japan
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Abstract: | The amniotic fluid contains mesenchymal stem cells (MSCs) and can be readily available for tissue engineering. Regenerative treatments such as tissue engineering, cell therapy, and transplantation show potential in clinical trials of degenerative diseases. Disease presentation and clinical responses in the Canis familiaris not only are physiologically similar to human compared with other traditional mammalian models but is also a suitable model for human diseases. The aim of this study was to investigate whether canine amniotic-fluid-derived mesenchymal stem cells (cAF-MSCs) can differentiate into neural precursor cells in vitro when exposed to neural induction reagent. During neural differentiation, cAF-MSCs progressively acquire neuron-like morphology. Messenger RNA (mRNA) expression levels of neural-specific genes, such as NEFL, NSE, and TUBB3 (βIII-tubulin) dramatically increased in the differentiated cAF-MSCs after induction. In addition, protein expression levels of nestin, βIII-tubulin, and tyrosine hydroxylase remarkably increased in differentiated cAF-MSCs. This study demonstrates that cAF-MSCs have great potential for neural precursor differentiation in vitro. Therefore, amniotic fluid may be a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases. |
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