Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging |
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| Authors: | D M Kurnit |
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| Institution: | Howard Hughes Medical Institute, Department of Pediatrics, University of Michigan Medical Center, Ann Arbor 48109-0650. |
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| Abstract: | I describe the construction of a variety of Escherichia coli recA deletion strains designed to facilitate molecular cloning. These recA deletion strains permit the efficient cloning of foreign inserts carried in plasmid, phage, cosmid, phasmid (phage-plasmid hybrid) or phosmid (phage-cosmid hybrid) vectors. |
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