| 盐生植物海马齿离体再生 |
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| 引用本文： | 申龙斌,段瑞军,郭建春,符少萍.盐生植物海马齿离体再生[J].植物学通报,2010,45(1):91-94. |
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| 作者姓名： | 申龙斌 段瑞军 郭建春 符少萍 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海口,571101;海南大学农学院,儋州,571737
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 973计划，中国热带农业科学院，中央级公益性科研院所基本科研项目 |
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| 摘 要： | 建立盐生植物海马齿（Sesuvium portulacastrum）的离体再生体系,为其生物技术改良奠定基础。以海马齿叶片、茎和腋芽为外植体, 在不同激素配比的培养基上进行愈伤组织诱导、继代培养以及不定芽的分化和生根培养。结果表明： 最适愈伤组织诱导的外植体为叶片, 其次为幼嫩的茎段和腋芽。以叶片为外植体, 愈伤组织诱导率最高的培养基为MS＋2.0mg·L^–12, 4-D ＋ 0.5 mg·L^–16-BA ＋ 3%sucrose; 芽分化最适培养基为MS ＋ 1.0 mg·L^–1 2, 4-D ＋ 0.2 mg·L^–1 6-BA ＋ 3% sucrose;生根最适培养基为MS ＋ 3%sucrose ＋ 0.1%AC。炼苗移栽后, 成活率可达80%。
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| 关 键 词： | 植株再生 海马齿 组织培养 |
Tissue Regeneration in Sesuvium portulacastrum |
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| Longbin Shen,Ruijun Duan,Jianchun Guo,Shaoping Fu Institute of Biotechnology , Bioscience,Chinese Academy of Tropical Agricultural Sciences,Haikou ,China College of Agriculture,Hainan University,Danzhou ,China.Tissue Regeneration in Sesuvium portulacastrum[J].Chinese Bulletin of Botany,2010,45(1):91-94. |
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| Authors: | Longbin Shen Ruijun Duan Jianchun Guo Shaoping Fu Institute of Biotechnology Bioscience Chinese Academy of Tropical Agricultural Sciences Haikou China College of Agriculture Hainan University Danzhou China |
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| Institution: | Longbin Shen1,2,Ruijun Duan1,Jianchun Guo1,Shaoping Fu1 1Institute of Biotechnology , Bioscience,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China 2 College of Agriculture,Hainan University,Danzhou 571737,China |
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| Abstract: | We aimed to develop a micropropagation protocol for regenerating tissue in halophilous Sesuvium portulacastrum and improving its basis for biotechnology study. The leaves, shoots and axillary buds were used as explants to induce calli, shoots and roots on different media. The highest efficiency of callus induction was from leaf disc, then stem and axillary bud. The optimal media for inducing calli, shoots and roots from leaves was a base of MS＋3% sucrose with 2.0 mg·L^–12, 4-D＋0.5 mg·L^–16-BA; 1.0 mg·L^–12, 4-D＋0.2 mg·L^–16-BA; and 0.1% active carbon, respectively. The survival rate of the cultured seedlings was up to 80% on soil. |
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| Keywords: | plant regeneration Sesuvium portulacastrum tissue culture |
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