Sarcoplasmic reticulum interacts with the Ca(2+) indicator precursor fura-2-am |
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| Authors: | S Highsmith P Bloebaum K W Snowdowne |
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| Affiliation: | 1. Centre for Advanced Materials & Industrial Chemistry (CAMIC), School of Science, RMIT University, Melbourne, VIC, Australia;2. School of Nano Sciences, Central University of Gujarat, Gandhinagar, India;1. Children''s Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China;2. Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China;3. University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518000, China |
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| Abstract: | Fura-2-am, the pentaester precursor of the fluorescent Ca(2+) indicator fura-2, is modified when it is exposed to isolated skeletal muscle sarcoplasmic reticulum vesicles. The modified fura-2-am has enhanced fluorescence, is not sensitive to Ca(2+), and is partially bound to the SR membrane. The isolated product is further converted into fura-2 by esterase. It is suggested that the SR-induced modification is a selective enzymatic hydrolysis of only some of the five ester moieties on fura-2-am. A structure is proposed to account for the results. The potential for this effect of SR on fura-2-am to cause complications in measurements of in vivo intracellular free [Ca(2+)] is noted. |
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