Conversion of oleic acid to 10-hydroxystearic acid by Nocardia paraffinae |
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| Authors: | A. Latrasse S. Paitier B. Lachot P. Bonnarme G. Féron A. Durand J.L. Le Quéré |
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| Affiliation: | (1) Laboratoire de Recherches sur les Aromes, Institut National de la Recherche Agronomique (INRA), 17 rue Sully, 21034 Dijon, France |
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| Abstract: | Resting cells of Nocardia paraffinaeCBS 255 58 anaerobically converted oleic acid to 10-hydroxystearic acid (optimum activity at 35°C and pH 7). Addition of oleic acid (0.22 mg/ml of medium), as an inducer, improved the hydroxylating activity. The biocatalyst was inactivated by sonication, lyophilization and heating, but kept 65% of its initial activity after five freeze-thawing treatments. This activity appeared to be located either in the cells or bound to the plasmic membrane. |
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