Isolation and Characterization of Cell Lines Resistant to Crude Culture Filtrate of Fusarium in Chickpea |
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| Authors: | Surya Parkash Anuradha Sehgal Randhir Singh J. B. Chowdhury |
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| Affiliation: | 1. Department of Genetics, CCS Haryana Agricultural University, Hissar, 125 004, India
2. Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hissar, 125 004, India
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| Abstract: | Cell lines resistant to Fusarium oxysporum f. sp. Ciceri crude culture filtrate have been selected In Cicer arietinum cv C235. Phenol content and the activities of tyrosine ammonia lyase (TAL), phenylalanine ammonia lyase (PAL) and ß-1, 3-glucanase were Increased by the elicitor In callus cultures of Fusarium susceptible cultlvar C235, resistant cultlvar WR315 and a stable Fusarium resistant cell clone FC-3. The Increase In phenols and glucanase activity, was more In selected cell line than In ev C235 and ev WR315, while the increase In activities of TAL and PAL showed reverse correlation. |
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