Molecular characterization of rotaviruses obtained from patients with rotavirus-associated encephalitis/encephalopathy |
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Authors: | Masaru Ihira Yoshiki Kawamura Hiroki Miura Fumihiko Hattori Yuki Higashimoto Ken Sugata Tomihiko Ide Satoshi Komoto Koki Taniguchi Tetsushi Yoshikawa |
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Affiliation: | 1. Faculty of Clinical Engineering, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan;2. Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan;3. Faculty of Medical Technology, Fujita Health University School of Medical Sciences, Toyoake, Aichi, Japan;4. Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan |
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Abstract: | Group A rotavirus (RVA) rarely causes severe complications such as encephalitis/encephalopathy. However, the pathophysiology of this specific complication remains unclear. Next-generation sequence analysis was used to compare the entire genome sequences of RVAs detected in patients with encephalitis/encephalopathy and gastroenteritis. This study enrolled eight patients with RVA encephalitis/encephalopathy and 10 with RVA gastroenteritis who were treated between February 2013 and July 2014. Viral RNAs were extracted from patients' stool, and whole-genome sequencing analysis was carried out to identify the specific gene mutations in RVA obtained from patients with severe neurological complications. Among the eight encephalitis/encephalopathy cases, six strains were DS-1-like G1P[8] and the remaining two were Wa-like G1P[8] (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Meanwhile, eight of the 10 viruses detected in rotavirus gastroenteritis patients were DS-1-like G1P[8], and the remaining two were Wa-like G1P[8]. These strains were further characterized by conducting phylogenetic analysis. No specific clustering was demonstrated in RVAs detected from encephalitis/encephalopathy patients. Although the DS-1-like G1P[8] strain was predominant in both groups, no specific molecular characteristics were detected in RVAs from patients with severe central nervous system complications. |
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Keywords: | encephalitis/encephalopathy molecular epidemiology next-generation sequencing rotavirus gastroenteritis RVA DS-1-like G1P[8] RVA Wa-like G1P[8] |
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