Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor |
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Authors: | Naoki Onoda Yukihiro Hiramatsu Shihono Teruya Koichiro Suzuki Yasuhiko Horiguchi |
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Institution: | 1. Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;2. Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
The Research Foundation for Microbial Diseases of Osaka University (BIKEN), Suita, Osaka, Japan |
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Abstract: | An autotransporter of Bordetella pertussis, virulence-associated gene 8 (Vag8), binds and inactivates the complement regulator, C1 inhibitor (C1-Inh), and plays a role in evasion of the complement system. However, the molecular interaction between Vag8 and C1-Inh remains unclear. Here, we localized the minimum region of Vag8 required for interaction with C1-Inh by examining the differently truncated Vag8 derivatives for the ability to bind and inactivate C1-Inh. The truncated Vag8 containing amino-acid residues 102–548, but not 102–479 and 202–648, showed the full activity of intact Vag8, suggesting that the separate 102–202 and 548–648 amino-acid regions of Vag8 mediate the interaction with C1-Inh. |
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Keywords: | Bordetella pertussis C1 inhibitor Vag8 |
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