Prevalence and genomic characterization of Group A Streptococcus dysgalactiae subsp. equisimilis isolated from patients with invasive infections in Toyama prefecture,Japan |
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| Authors: | Haruka Ishihara Kohei Ogura Tohru Miyoshi-Akiyama Masahiko Nakamura Hiroyasu Kaya Shigefumi Okamoto |
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| Affiliation: | 1. Department of Clinical Laboratory Science, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan;2. Advanced Health Care Science Research Unit, Institute for Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, Japan;3. Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan;4. Department of Medical Laboratory, Toyama Prefectural Central Hospital, Toyama, Toyama, Japan;5. Department of Internal Medicine, Toyama Prefectural Central Hospital, Toyama, Toyama, Japan |
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| Abstract: | Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive streptococcal infections, especially in elderly people. Between 2013 and 2018, 88 streptococci were isolated from clinical blood culture in a hospital in Toyama prefecture, Japan. The collection included six Group A SDSE (ASD) strains, which are rarely isolated. Multilocus sequence typing categorized five of the six strains into ST128 and the remaining strain into a new type. Maximum-likelihood phylogenetic analysis revealed that the six ASD strains had highly similar genome sequences. Bayesian analysis indicated that the most recent common ancestor of the strains appeared 39 years ago. The ASD strains possessed carbohydrate synthase genes that are conserved in Streptococcus pyogenes strains, whereas one strain featured a different arrangement of the gene cluster. The carbohydrate synthase genes varied by Lancefield type (A, C, and G). |
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| Keywords: | bacteremia Bayesian analysis Lancefield Group A Streptococcus dysgalactiae subsp. equisimilis whole genome sequencing |
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