| Institution: | 1. Bioinformatics Centre, Savitribai Phule Pune University, Pune, Maharashtra, India
Physical Chemistry Division, National Chemical Laboratory, Pune, Maharashtra, India;2. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, India;3. Physical Chemistry Division, National Chemical Laboratory, Pune, Maharashtra, India;4. Bioinformatics Centre, Savitribai Phule Pune University, Pune, Maharashtra, India |
| Abstract: | Thionins are small, cysteine-rich peptides that play an important role in plant defense, primarily through their interactions with membranes. Eight novel γ-thionin peptides (CanThio1-8) were isolated from the flower of Capsicum annuum. Sequence analysis revealed that the peptides cluster into three groups. A representative peptide from each group (CanThio1, 2, and 3) was used for experimental characterization. Interestingly, peptides were found to possess some cytotoxic activity against normal human embryonic kidney cell line but higher cytotoxicity against cancer cell line MCF-7. CanThio3 peptide was chosen as a representative peptide to study the molecular mechanism of action on membranes. Microsecond timescale atomistic simulations of CanThio3 were performed in the presence of a POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) lipid bilayer. Simulations revealed that CanThio3 interacts with the bilayer and causes lipid thinning in the vicinity. Nonpolar amino acids specific to the α-core region of CanThio3 along with nonpolar residues in the γ-core region are seen to interact with the lipid tails. The differences in the amino acid sequence of CanThio peptides in these regions explain the variability in cytotoxic activities. In summary, our results demonstrate the membrane-mediated activity of a novel series of γ-thionin peptides from C. annuum. |
