Quantitative trait loci mapping of adult-plant resistance to powdery mildew in triticale |
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| Authors: | Ma?gorzata Karbarz Miros?aw Tyrka Anna Wo?niak-Strzembicka Grzegorz Czajowski Agnieszka Wardyńska Dorota Tyrka Miros?aw Pojmaj Maria W?dzony |
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| Institution: | 1. Department of Botany, Faculty of Biotechnology, University of Rzeszow, Rzeszow, Poland;2. Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland;3. Cereal Department, Plant Breeding and Acclimatization Institute, Krakow, Poland;4. Department of Plant Breeding and Genetics, Laboratory of Applied Genetics, Plant Breeding and Acclimatization Institute, Radzikow, Poland;5. Laboratory of Population Genetics, Polonia University, Czestochowa, Poland;6. DANKO Plant Breeding Ltd., Choryn, Poland;7. Institute of Biology, Faculty of Geography and Biology, Pedagogical University of Krakow, Krakow, Poland |
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| Abstract: | Powdery mildew (PM) is a common disease caused by Blumeria graminis, which affects cereals and has recently adapted to triticale. Adult-plant resistance (APR) genes provide durable protection of crops from the disease. Quantitative trait loci corresponding to the APR effects were mapped in an F2 population of “Lamberto” (susceptible) × “Moderto” (resistant). A genetic map of winter triticale was constructed based on the segregation of 863 DArT, 38 microsatellite and 10 resistance gene analogue markers. Composite interval mapping (CIM) was applied to identify three QTLs for maximum disease severity (MDS) and two for the area under disease progress curve (AUDPC) conferring resistance to the powdery mildew on chromosomes: 6A, 7A, 1B and 4R. The 39% variation in AUDPC was explained by the main QTL localised on chromosome 4R. Genes coding TRIUR3 proteins, serine/threonine protein kinase and cell wall associated kinases were localised in silico within the QTL and alternative DNA markers were proposed for flexible use in laboratories of diversified throughput. |
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| Keywords: | genetic map molecular marker powdery mildew quantitative trait locus triticale |
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