莱茵衣藻磷脂二脂酰甘油酰基转移酶3在三酰甘油合成中的功能研究

为研究磷脂二脂酰甘油酰基转移酶（PDAT）在三酰甘油合成中的功能，克隆了莱茵衣藻（Chlamydomonas reinhardtii） PDAT同源基因CrPDAT3干涉片段，通过构建CrPDAT3 RNAi 干涉载体并转化莱茵衣藻，对CrPDAT3基因有效沉默，结果显示转基因藻株生长减缓，油脂含量下降14.65%-45.15%，说明CrPDAT3对油脂合成起到重要的作用。研究结果对于该基因应用于微藻油脂的遗传改良将起到重要作用。  

THE ROLE OF PHOSPHOLIPID: DIACYLGLYCEROL ACYLTRANSFERASE IN BIOSYNTHESIS OF TRIACYLGLYCEROL BY CHLAMYDOMONAS REINHARDTII

Currently, production of biodiesel by microalgae has been regarded as a promising source of renewable energy. However, the understanding of oil biosynthesis mechanisms in micro-algae is limited. Phospholipid: diacylglycerol acyltransferase catalyzes phospholipid and diacylglycerol to produce triglyceride, a key reaction in triglyceride synthesis. In this study, we cloned a fragment of Phospholipid: diacylglycerol acyltransferase homologous gene 3 CrPDAT3 in Chlamydomonas, which was then used to construct a CrPDAT3 RNAi interference vector and transferred into Chlamydomonas. The results showed that the growth rate of transgenic algae strain was declined. At the same time, the oil content was decreased by 14.65%-45.15%, showing that the CrPDAT3 playing an important role in oil biosynthesis.