不同营养方式对小球藻FACHB 484生长的影响及其非自养生长机理研究

系统研究了小球藻FACHB 484在含有葡萄糖的不同营养方式下的生长情况，并通过抑制试验探讨葡萄糖在小球藻FACHB 484光异养和兼养生长条件下所起的作用以及小球藻FACHB 484是否存在氧化呼吸系统的关键酶类。结果表明:小球藻FACHB 484可利用葡萄糖进行化能异养、光激活异养、光异养及兼养生长，其生长速率大小为:兼养光异养光激活异养化能异养光合自养。兼养培养的最大生物量和比生长速率分别是自养培养的8.6和3.4倍，其比生长速率接近于光合自养和光异养培养下的比生长速率之和。葡萄糖主要作为小球藻FACHB 484兼养和光异养培养的碳源，而能量主要源自光。小球藻FACHB 484存在氧化呼吸链代谢途径，其细胞中有琥珀酸脱氢酶和细胞色素氧化酶。  

STUDY ON THE EFFECTS OF DIFFERENT NUTRITION MODES ON THE GROWTH OF CHLORELLA SP. FACHB 484 AND THE MECHANISM OF HETEROTROPHIC GROWTH

Here we systematically studied the growth of Chlorella sp. FACHB 484 in glucose-containing medium in different nutrition modes. We also conducted the inhibition tests to investigate the effects of glucose on the growth of Chlorella sp. FACHB 484 in heterotrophic and mixotrophic conditions, as well as to identify the key enzyme in oxidation respiratory system. We observed that Chlorella sp. FACHB 484 could utilize glucose as the source of nutrition in all the test nutritional modes and showed different growth rates in the order mixotrophy photoheterotrophy light-activated heterotrophy chemoheterotrophy photoautotrophy. The maximum biomass and specific growth rate (SGR) of the tested algae in mixotrophy were 8.6 and 3.4 times higher than those in photoautotrophy. The value of SGR of Chlorella sp. FACHB 484 in mixotrophy was close to the sum of the values of SGRs in photoautotrophy and photoheterotrophy. In mixotrophic and photoheterotrophic modes, Chlorella sp. FACHB 484 used glucose and light as the sources of carbon and energy respectively. The cells of Chlorella sp. FACHB 484 expresses succinic dehydrogenase and cytochrome oxidase which are most likely the key enzymes of the oxidation respiratory chain.