生物条形码检测技术及其研究进展

生物条形码检测技术作为一种新的诊断工具,已逐渐应用于蛋白质、核酸和小分子物质的检测.生物条形码技术通过构建"纳米金颗粒-目标物-磁纳米颗粒"三明治结构,利用磁场作用,将结合在纳米金颗粒表面的大量相同序列的寡聚核苷酸洗脱下来,并进一步放大信号,实现对目标物的间接或直接检测.本文介绍了生物条形码检测技术的原理及其应用,综述...

Detection technology and research progress of biological barcode

Bio-barcode technology, as a new diagnostic tool, has been gradually applied to the detection of proteins, nucleic acids, and small-molecule compounds. Based on the sandwich structure of gold nanoparticles and using magnetic field effects, the bio-barcode technology aims to amplify the signal after eluting numerous oligonucleotides of the same sequence on the surface of gold nanoparticles and complete the detection of the target directly and indirectly. In this study, the principle and application of bio-barcodes are introduced, and the joint application of bio-barcode and biochip silver-dyeing technology, enzyme-labeled gold nanoscale technology, biosensor technology, microplate silver-dyeing technology, and polymerase chain reaction technology are reviewed. Considering the future outlook of this technology, it is of great significance to explore the best reaction conditions, optimize the operation procedures, explore the residue detection of a variety of substances, and develop standardized and commercial immunity kits for bio-barcode technology in the future.