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Carboxylesterase activities during the establishment of the procambium and procortex in embryos of pisum sativum L.
Authors:D. F. Carmignac  P. B. Gahan  Z. Loukopoulou
Abstract:A cytochemical and electrophoretic analysis of alpha-naphthyl and naphthol AS-D acetate esterases from the radicles of pea seeds germinated for 24, 36 and 48 h permitted the detection of the loss of activity from the procortex with time after germination, but a stability of this activity in the prostele. Inhibitor studies showed the procambial enzyme as a single band of carboxylesterase activity the remainder being acetylesterase activity. The procambial carboxylesterase which is present in the more mature root apex corresponds to that of the 48-h embryo (separating at Rf 0·74).
Keywords:Pisum sativum  embryo  germination  carboxylesterases  isozyms  cytodifferentiation  cytochemistry  gel electrophoresis
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