线粒体促凋亡因子Omi/HtrA2在肝癌组织中表达的研究

目的探讨丝氨酸蛋白酶Omi/HtrA2在肝癌组织、癌旁组织与正常肝组织中的表达及意义。方法应用免疫组化SABC法检测43例肝癌、30例癌旁组织及10例正常肝组织中Omi/HtrA2的表达。结果29例(67·44%)肝癌中Omi/HtrA2蛋白表达阳性,30例癌旁组织和10正常肝组织没有或只有少量很弱的表达。肝癌高分化组中Omi/HtrA2蛋白的表达明显高于中、低分化组(P<0·01)。另外,Omi/HtrA2表达与肿瘤大小及临床分期相关,但Omi/HtrA2表达与肝硬化、有无癌栓、HBsAg和AFP无关。结论肝细胞癌可能需要Omi/HtrA2的表达来促进凋亡,Omi/HtrA2的表达对肝癌的发展有重要作用。

STUDY OF OMI/HTRA2 EXPRESSION IN HEPATOCELLULAR CARCINOMA

Objective To investigate the expression and clinical significance of the serine protease Omi/HtrA2 in hepatocellular carcinoma, paracancerous tissues and normal hepatic tissues. Methods The expression of Omi/HtrA2 was assayed by immunohistochemical means in 43 hepatocellular carcinoma, 30 paracancerous tissue and 10 normal hepatic tissue specimens. And its relation with clinical pathological characteristics was analysed too. Results The positive rate of Omi/HtrA2 expression in the tissues of hepatocellular carcinoma was 67.44%, which was significantly higher than that in paracancerous tissues and normal hepatic tissues (P< 0. 01). The positive rate of Omi/HtrA2 was higher in the well differentiated group than that in poorly and moderately differentiated groups (P< 0. 01). The cells in paracancerous tissue and normal hepatic tissue groups showed no or weak expression of Omi/HtrA2. In addition, the Omi/HtrA2 expression in hepatocellular carcinoma showed a correlation with tumor size and clinical stages (P< 0. 05), but no correlation with portal vein embolus, live cirrhosis and the status of HBsAg and AFP (P> 0. 05). Conclusion Hepatocellular carcinoma may need Omi/HtrA2 expression for apoptosis, and Omi/HtrA2 expression might play an important role in the development of hepatocellular carcinoma.