Molecular diversity of microbial community in acid mine drainages of Yunfu sulfide mine |
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Authors: | Zhiguo He Shengmu Xiao Xuehui Xie Hui Zhong Yuehua Hu Qinghua Li Fenglin Gao Guiyuan Li Jianshe Liu Guanzhou Qiu |
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Institution: | (1) School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, China;(2) Cancer Research Institute, Central South University, Changsha, 410078, China |
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Abstract: | Two acid mine drainage (AMD) samples were studied by a PCR-based cloning approach, which were from Yunfu sulfide mine in Guangdong
province, China. A total of 15 operational taxonomic units (OTUs) were obtained from the two AMD samples. The percentage of
overlapped OTUs in two AMD samples was 42.1%. Phylogenetic analysis revealed that the bacterium in the two samples fell into
four putative divisions, which were Nitrospira, α-Proteobacteria, β-Proteobacteria, and γ-Proteobacteria four families. Organisms of genuses Acidithiobacillus and Gallionella, which were in γ-Proteobacteria family and β-Proteobacteria family, respectively, were dominant in two samples. The proportions of clones affiliated with Gallionella in each sample were 47.2% (G2) and 16.9% (G1). The result suggested that organisms of Gallionella were a very important composition in microbial communities of the two AMD samples we studied. In addition, the PCR amplification
of archaeal 16S rDNA genes form these two AMD samples have been performed with two sets of archaea-specific primers, but no
PCR product found.
Zhiguo He, Shengmu Xiao, Xuehui Xie, and Hui Zhong equally contributed to this work. |
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Keywords: | Microbial diversity Ecology RFLP AMD |
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