| 小麦叶片愈伤组织及其再生植株的诱导 |
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| 引用本文： | 王万军,王文芳.小麦叶片愈伤组织及其再生植株的诱导[J].西北植物学报,1998,18(3):401-405. |
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| 作者姓名： | 王万军 王文芳 |
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| 作者单位： | 陕西省中国科学院植物研究所 |
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| 摘 要： | 小麦幼苗基部外植体在补加２，４－Ｄ的ＭＳ、Ｎ６、ＢＡ１（３）培养基上均可诱导出愈伤组织，２，４－Ｄ的最适浓度为２．０ｍｇ／Ｌ；愈伤组织的增殖速度与切段部位及基本培养基有关，其中在以ＭＳ补加２．０ｍｇ／Ｌ２，４－Ｄ的培养基上诱导出的愈伤组织增长速度最快；最后讨论了小麦愈伤组织幼苗诱导率低的原因及可能解决的方法。
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| 关 键 词： | 小麦 叶片 愈伤组织 再生植株 |
THE INDUCTION OF CALLUS AND PLANTLET REGENERATION FROM THE LEAF OF WHEAT ( TRITICUM AESTIVUM L.) |
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| Wang Wanjun,Wang Wenfang,Cao Jianjun and Bai Shouxin.THE INDUCTION OF CALLUS AND PLANTLET REGENERATION FROM THE LEAF OF WHEAT ( TRITICUM AESTIVUM L.)[J].Acta Botanica Boreali-Occidentalia Sinica,1998,18(3):401-405. |
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| Authors: | Wang Wanjun Wang Wenfang Cao Jianjun and Bai Shouxin |
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| Abstract: | Calli are induced from the explants of wheat younger seedlings in the MS,N 6,BN 1(3) media supplemented with 2,4 D.The best concentration of 2,4 D is 2.0 mg/L.The growing speed of calli are related with the position of segments and the basic medium.We obtained the highly growing spead of calli in the MS medium supplemented with 2.0 mg/L.2,4 D.The reason for low induction rate of seedlings from leaf derived calli and the resolving method are discussed. |
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| Keywords: | wheat leaf callus plant regeneration |
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