The arrangement of DNA in lambda phage heads. I. Biological consequences of micrococcal nuclease attack on a portion of the chromosome exposed in tailless heads |
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| Authors: | V C Bode F D Gillin |
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| Affiliation: | 1. Department of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea;2. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;3. Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of Korea;4. Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea;5. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;6. Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea |
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| Abstract: | If they lack tails, lambda heads are sensitive to inactivation by micrococcal DNase. Nuclease-treated heads still join with tails, producing phage which adsorb to host cells and inject their DNA normally. The injected chromosomes are defective since both lytic phage production and lysogeny are decreased 85 to 95%. The intracellular circularization of λ DNA is the first step in phage development altered by nuclease damage. When extracted from an infected cell, most parental molecules from nuclease-treated heads are of normal size but linear. The failure to cyclize in vivo suggests the cohesive ends are damaged. |
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