The role of ocelli in circadian singing rhythms of crickets

ABSTRACT. . There is a direct quantitative relationship between the free-running period (r) of the circadian stridulation rhythm of male Australian field crickets, Teleogryllus commodus (Walker), and the intensity of the constant light conditions. Both T. commodus and the house cricket, Acheta domesticus (L.), show free-running periods of the singing rhythm of c. 24 h when the light intensity is 0.00025 lux. In both species the severance of the three ocellar nerves significantly slows the circadian period which is indicative of a reduced perception of the available light intensity. To test whether this period reduction is a peripheral or a central effect, electro-retinograms (ERGs) were recorded from compound eyes of male T. commodus with the ocelli fully functional, then occluded, and then uncovered. The size of the compound eye ERG is reduced by 20% with ocellar occlusion and can subsequently be fully restored to the intact level, which indicates that the ocellar effect is a peripheral one. Intensified CoCl₂ fills reveal one neurone in A. domesticus and two in T. commodus which travel from the lateral ocellar nerve out into the ipsilateral optic lobe of the compound eye. These neurones all terminate in or distal to the lobular neuropile. The data are interpreted to indicate a role for ocelli in modulating the light intensity perception of the compound eye. The final effect of the ocellar afferents is at a peripheral level prior to the input of the visual information to the optic lobe circadian pacemaker. Hence ocelli play an indirect role in circadian rhythmicity, augmenting the sensitivity of the primary photoreceptors to better perceive photic entrainment signals.