| 地衣芽胞杆菌DW2中敲除氨基酸转运蛋白基因yhdG提高杆菌肽产量 |
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| 引用本文： | 李阳,吴非,蔡冬波,占杨杨,李俊辉,陈晓斌,陈慧超,陈守文,马昕.地衣芽胞杆菌DW2中敲除氨基酸转运蛋白基因yhdG提高杆菌肽产量[J].生物工程学报,2018,34(6):916-927. |
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| 作者姓名： | 李阳 吴非 蔡冬波 占杨杨 李俊辉 陈晓斌 陈慧超 陈守文 马昕 |
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| 作者单位： | 湖北大学生命科学学院生物资源绿色转化湖北省协同创新中心湖北省环境微生物工程技术研究中心;绿康生化股份有限公司 |
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| 基金项目： | 国家重点基础研究发展计划 (973计划) (No. 2015CB150505) 资助。 |
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| 摘 要： | 杆菌肽是微生物产生的由11种氨基酸残基组成的广谱性抗生素,前体物的供应可能是限制杆菌肽高产的重要因素。文中通过支链氨基酸(异亮氨酸、亮氨酸、缬氨酸)的添加实验考察了前体物质支链氨基酸对杆菌肽高产的影响,证实了异亮氨酸(Ile)和亮氨酸(Leu)的添加可以提高杆菌肽的效价,其中Ile的添加对杆菌肽效价提高的效果较为明显。随后,文中以地衣芽胞杆菌DW2为出发菌株,分别构建了支链氨基酸转运蛋白Yhd G的缺失和强化表达菌株。发酵结果表明,转运蛋白Yhd G缺失工程菌DW2△yhd G的杆菌肽效价达到917.35 U/m L,与原始菌DW2相比提高了11%,而强化Yhd G则会使杆菌肽效价下降25%。最后通过分析胞内胞外支链氨基酸含量,发现缺失转运蛋白Yhd G能够在发酵中后期显著提高胞内支链氨基酸含量,表明氨基酸转运蛋白Yhd G在地衣芽胞杆菌DW2中可能发挥着氨基酸输出的功能。综上,文中通过缺失转运蛋白Yhd G显著提高了地衣芽胞杆菌胞内支链氨基酸的供给水平,从而提高了杆菌肽效价,为杆菌肽高产菌株的构建提供了一种新的策略。
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| 关 键 词： | 杆菌肽，地衣芽胞杆菌，支链氨基酸，转运蛋白YhdG |
| 收稿时间： | 2017/12/18 0:00:00 |
Enhanced production of bacitracin by knocking out of amino acid permease gene yhdG in Bacillus licheniformis DW2 |
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| Yang Li,Fei Wu,Dongbo Cai,Yangyang Zhan,Junhui Li,Xiaobin Chen,Huichao Chen,Shouwen Chen and Xin Ma.Enhanced production of bacitracin by knocking out of amino acid permease gene yhdG in Bacillus licheniformis DW2[J].Chinese Journal of Biotechnology,2018,34(6):916-927. |
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| Authors: | Yang Li Fei Wu Dongbo Cai Yangyang Zhan Junhui Li Xiaobin Chen Huichao Chen Shouwen Chen and Xin Ma |
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| Institution: | 1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China,1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China,1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China,1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China,2 Lifecome Biochemistry Co. Ltd, Pucheng 353400, Fujian, China,2 Lifecome Biochemistry Co. Ltd, Pucheng 353400, Fujian, China,2 Lifecome Biochemistry Co. Ltd, Pucheng 353400, Fujian, China,1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China and 1 Environmental Microbial Technology Center of Hubei Province, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China |
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| Abstract: | |
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| Keywords: | bacitracin Bacillus licheniformis branched chain amino acids amino acid permease YhdG |
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