| 鸡GM-CSF和IL-2增强表达新城疫病毒F蛋白的重组杆状病毒疫苗的免疫效果 |
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| 引用本文： | 于航,高冬妮,申燕,刘颖,平文祥,葛菁萍.鸡GM-CSF和IL-2增强表达新城疫病毒F蛋白的重组杆状病毒疫苗的免疫效果[J].生物工程学报,2018,34(9):1442-1452. |
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| 作者姓名： | 于航 高冬妮 申燕 刘颖 平文祥 葛菁萍 |
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| 作者单位： | 黑龙江大学生命科学学院微生物省高校重点实验室 |
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| 基金项目： | 国家自然科学基金 (No. 31270143)，黑龙江省高等学校科技创新团队“农业微生物发酵技术” (No. 2012td009) 资助。 |
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| 摘 要： | 为比较鸡粒细胞-巨噬细胞集落刺激因子(Granulocyte macrophage colony stimulating factor,GM-CSF)及鸡白细胞介素2(Interleukin 2,IL-2)对杆状病毒疫苗的免疫增强效果,通过基因工程手段构建重组杆状病毒疫苗(Recombinant Baculovirus,BV)rBV-LMI-F,并联合GM-CSF及IL-2进行鸡体免疫。对中和抗体水平及细胞因子含量比较GM-CSF和IL-2的免疫增强效果进行对比。结果显示,在第一次免疫28 d或42 d后,GM-CSF联合免疫组可诱导鸡体产生更高的抗体和细胞因子水平(P0.01)。表明鸡GM-CSF能更有效地刺激机体产生较强的抗体和细胞因子反应,提高重组杆状病毒疫苗的免疫效果。
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| 关 键 词： | 新城疫，GM-CSF，IL-2，联合免疫，免疫效果 |
| 收稿时间： | 2017/12/21 0:00:00 |
Efficacy enhancement of a Baculovirus-vectored Newcastle Disease Virus F protein vaccine by chicken GM-CSF and IL-2 |
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| Hang Yu,Dongni Gao,Yan Shen,Ying Liu,Wenxiang Ping and Jingping Ge.Efficacy enhancement of a Baculovirus-vectored Newcastle Disease Virus F protein vaccine by chicken GM-CSF and IL-2[J].Chinese Journal of Biotechnology,2018,34(9):1442-1452. |
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| Authors: | Hang Yu Dongni Gao Yan Shen Ying Liu Wenxiang Ping and Jingping Ge |
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| Institution: | Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China,Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China,Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China,Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China,Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China and Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China |
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| Abstract: | To compare with the effects of the GM-CSF and IL-2 used as adjuvants in the baculovirus vaccine, we used genetic engineering to construct the recombinant baculovirus rBV-LMI-F and with GM-CSF and IL-2 to immunized chickens. Then, we compared the concentration of the neutralizing antibody and cytokines to determine the immunostimulatory effects of GM-CSF and IL-2. GM-CSF induced higher levels of antibodies and cytokines in chickens at 28 d and 42 d post-vaccination. In conclusion, GM-CSF could elicit higher serum antibody and cytokines responses and improved the effects of Baculovirus vaccine. |
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| Keywords: | Newcastle disease GM-CSF IL-2 co-immunization immune effect |
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