Simplified validation of borderline hits of database searches |
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Authors: | Thomas Henrik Shevchenko Andrej |
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Affiliation: | Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. |
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Abstract: | Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a simple software tool, display the candidate sequences together with each database search hit. We demonstrate that comparing hit database sequences and independent de novo interpretations of the same MS/MS spectra assists in rapid examination of ambiguous matches. |
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Keywords: | De novo sequencing Hit validation MASCOT MS BLAST MS/MS |
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