A New Stain for Pollen Fertility Studies |
| |
Authors: | P. H. Ramanjini Gowda Thilak K. Subbaiah S. H. Joshi |
| |
Affiliation: | a Division of Horticulture and Department of Basic Botany, Bangalore, India |
| |
Abstract: | The juice from the berries of Cocculus hirsutum was extracted and used for pollen fertility studies in various crops. Two stains were prepared: P. H. Ramanjini (PHR) stain and modified PHR stain. The modified PHR stain contains lactic acid and produces the best staining differentiation. The intensity of the staining was dependent on the thickness of the pollen cell walls, hence PHR stain is recommended for thick walled pollen grains and the modified PHR stain for pollen with relatively thin walls. The preparation of both the stains are very simple, quick and inexpensive. |
| |
Keywords: | Cocculus hirsutum PHR stain modified PHR stain pollen fertility |
本文献已被 InformaWorld 等数据库收录! |