Chemical permeabilization and in situ removal of daidzein from biologically viable soybean (Glycine max) seeds |
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Authors: | Kittinan Komolpis Peter B. Kaufman Henry Y. Wang |
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Affiliation: | (1) Department of Chemical Engineering, The University of Michigan, Ann Arbor, USA;(2) Department of Biology, The University of Michigan, Ann Arbor, USA |
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Abstract: | After 24 h of chemical permeabilization with 20% (v/v) methanol at 25 °C, the amount of daidzein released from soybean seeds is 15 to 20% of the amount (0.0423 ± 0.0045 mg/g seed dry wt) obtained by physical grinding. With this chemical permeabilization condition, 70% of the permeabilized seeds are still able to germinate. The release of daidzein is enhanced to 33% with the addition of XAD-4 to 20% (v/v) methanol without affecting seed viability. © Rapid Science Ltd. 1998 |
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Keywords: | chemical permeabilization in situ product removal daidzein soybean |
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