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Restriction site map of the Chlorella virus PBCV-1 genome
Authors:Lois E Girton  James L van Etten
Institution:(1) Department of Plant Pathology, University of Nebraska, 68583-0722 Lincoln, NE, USA;(2) Present address: Department of Botany, Iowa State University, 50011 Ames, IA, USA
Abstract:The virus PBCV-1, which replicates in a Chlorella-like green alga, has a dsDNA genome. The DNA was mapped for BamHI, HindIII, and PstI restriction sites. The resulting map has a size of 333 kbp and is circular—indicating either covalently closed circular DNA or circularly permuted linear DNA. Several regions of repetitive DNA were also identified and located on the restriction map.
Keywords:Chlorella virus dsDNA genome  restriction map of PBCV-1 genome
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