Abstract: | We report for the first time that cultured lens epithelial celllayers and rabbit lenses in vitro transport fluid. Layers of the TN4mouse cell line and bovine cell cultures were grown to confluence onpermeable membrane inserts. Fluid movement across cultured layers andexcised rabbit lenses was determined by volume clamp (37°C).Cultured layers transported fluid from their basal to their apicalsides against a pressure head of 3 cmH2O. Rates were (inµl · h1 · cm2)3.3 ± 0.3 for TN4 cells (n = 27) and 4.7 ± 1.0 for bovine layers (n = 6). Quinidine, a blocker ofK+ channels, andp-chloromercuribenzenesulfonate andHgCl2, inhibitors of aquaporins,inhibited fluid transport. Rabbit lenses transported fluid from theiranterior to their posterior sides against a2.5-cmH2O pressure head at 10.3 ± 0.62 µl · h1 · lens1(n = 5) and along the same pressurehead at 12.5 ± 1.1 µl · h1 · lens1(n = 6). We calculate that this flowcould wash the lens extracellular space by convection about once every2 h and therefore might contribute to lens homeostasis and transparency. |