随机扩增多态性DNA技术对结核分支杆菌菌株鉴别及其耐药相关性分析

用引物G1和L1扩增的16S-23SrDNA间隔区作为模板DNA，再用引物AP50进行随机扩增多态性DNA（Random Amplified PolymorphicDNA,RAPD)分棉线AP50扩增出的各菌株DNA片段，用1.2%的琼脂糖凝胶电泳EB染色，可获得1或2个大小不同的DNA片段（390-1031bp)。各株间差异明显，结果表明，不同菌株有不同的DNA多态性，药物敏感株与耐药株之间多态性差异明显，耐药株中耐药谱相似或相同的菌株间其多态性也相似，RAPD有助于简便，快速，有效地了解菌株间的克隆相关性及耐药性传播。

Strains Differentiation and Analysis on Resistant Relation of Mycobacterium Tuberculosis by Random Amplified Polymorphism DNA

Amplification of the region separating the genes coding for 16?S and 23?S rDNA was performed with primers G1 and L1. Those PCR products were then used as the target DNA for random amplified polymorphism DNA (RAPD) analysis with arbitrary primer AP50. After RAPD with AP50, one or two DNA fragments with different sizes (390 to 1?031 bp) were separated using electrophoresis with EB stain from each strain. The difference is significant among strains. So it can be concluded that different strains had different DNA polymorphism, which are significant between drug-sensitive and drug-resistant strains. Drug-resistant strains with similar or common antibiotic profile had similar DNA polymorphism. Thus, RAPD is helpful for the simple, rapid and efficient investigation of colonal relation among strains and spread of antibiotic resistance.