| 从拟南芥表达文库中筛选钙调素结合蛋白cDNA克隆 |
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| 引用本文： | 李国亮,詹红利,李翠凤,刘华,凌启阆,Ren Zhng. 从拟南芥表达文库中筛选钙调素结合蛋白cDNA克隆[J]. 植物生理与分子生物学学报, 2001, 27(1): 28-32 |
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| 作者姓名： | 李国亮 詹红利 李翠凤 刘华 凌启阆 Ren Zhng |
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| 作者单位： | 1. 南开大学生物化学与分子生物学系，天津 300071
2. Department of Biological Sciences, University of Wollongong , Australia |
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| 基金项目： | 国家自然科学基金委资助项目(批准号：39670164)。 |
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| 摘 要： | 以生物素标记的钙调素为探针，筛选拟南芥λZAPⅡ表达文库，分离得到9个阳性克隆。融合蛋白的Westem印迹分析表明，这些阳性克隆确是编码钙调素结合蛋白的(图2，3)，并且其中Y9等8个克隆编码的融合蛋白与钙调素有依赖于钙离子的结合(图3B)；而唯独Y7编码的融合蛋白与钙调素的结合，与CaMBP-10一样，不依赖于钙离子的存在(图3A)。
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| 关 键 词： | 生物素标记钙调素，钙调素结合蛋白，表达文库筛选 |
| 修稿时间： | 1999-12-14 |
Cloning of cDNAs EncodingCalmodulin-binding Protein from Arabidop-sis cDNA Expression Library |
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| LI Guo Liang ZHAN Hong Li LI Cui Feng LIU Hua LING Qi Lang. Cloning of cDNAs EncodingCalmodulin-binding Protein from Arabidop-sis cDNA Expression Library[J]. Journal Of Plant Physiology and Molecular Biology, 2001, 27(1): 28-32 |
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| Authors: | LI Guo Liang ZHAN Hong Li LI Cui Feng LIU Hua LING Qi Lang |
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| Abstract: | Using protein-protein interaction,an Arabidopsis λZAP Ⅱ cDNAexpression library was screened with biotinylated calmodulin as a probe. Nine positive clones were isolated.The results of Western blotting of fusion proteins indicated that all these positive clones could synthesize peptides which could bind calmodulin ( Figs. 2,3). Eight of them bound calmodulin depending on the presence of Ca2+( Fig. 3 B), but only one fusion protein encoded by Y7 cDNA clone, which was the same as CaM BP-10, could bind CaM in a way independent of Ca2+ ( Fig. 3A). |
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| Keywords: | biotinylated CaM calmodulin binding protein expression library screening |
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