Tubules of the trans Golgi apparatus visualized by immunoelectron microscopy |
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Authors: | J Roth Douglas J Taatjes |
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Institution: | (1) Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland e-mail: juergen.roth@pty.usz.ch Tel.: +41 1 255 50 90; Fax: +41 1 255 44 07, CH;(2) Department of Pathology and Cell Imaging Facility, College of Medicine, University of Vermont, Burlington, VT 054405, USA, US |
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Abstract: | Tubules constitute an integral part of the Golgi apparatus and have been shown to form a complex and dynamic network at its
trans side. We have studied in detail structural features of the trans Golgi network and its relationship with the cisternal
stack in thin sections of Lowicryl K4M embedded human absorptive enterocytes by immunolectron microscopy. Immunoreactive sites
for α1,3 N-acetylgalactosaminyltransferase and blood group A substance were detectable troughout the cisternal stack and the
entire trans Golgi network. Furthermore, the entire trans Golgi network was reactive for CMPase activity. Evidence for two
kinds of tubules at the trans side of the Golgi apparatus was found: tubules that laterally connect adjacent and distant cisternal
stacks, and others extending from central and lateral portions of trans cisternae to form the complex and extensive trans
Golgi network. Trans cisternae showed often the peeling-off phenomenon and were continuous with the trans Golgi network. Both,
trans cisternae and tubules of the trans Golgi network exhibited regionally buds and vesicles with a lace-like, non clathrin
coat, previously reported by others in NRK cells, which contained glycoproteins with terminal N-acetylgalactosamine residues.
These buds and vesicle are therefore involved in constitutive exocytosis.
Accepted: 12 January 1998 |
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