A sulfated proteoglycan is necessary for storage of exocrine secretory proteins in the rat parotid gland |
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Authors: | Venkatesh S G Gorr S-U |
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Institution: | Department of Periodontics, Endodontics, and Dental Hygiene, University of Louisville Health Sciences Center, Louisville, Kentucky 40292, USA. |
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Abstract: | Sulfated proteoglycans have beenproposed to play a role in the sorting and storage of secretoryproteins in exocrine secretory granules. Rat parotid acinar cellsexpressed a 40- to 60-kDa proteoglycan that was stored in secretorygranules. Treatment of the tissue with the proteoglycan synthesisinhibitor paranitrophenyl xyloside resulted in the complete abrogationof the sulfated proteoglycan. Pulse-chase experiments in the presenceof the xyloside analog showed a significant reduction in the stimulatedsecretion and granule storage of the newly synthesized regulatedsecretory proteins amylase and parotid secretory protein. Inhibition ofproteoglycan sulfation by chlorate did not affect the sorting of theseproteins. The effect of proteoglycan synthesis inhibition on proteinsorting was completely reversed upon treatment with a weak acid. These results suggest that the sulfated proteoglycan is necessary for sortingand storage of regulated secretory proteins in the exocrine parotidgland. Preliminary evidence suggests that the mechanism involves themodulation of granule pH by the proteoglycan rather than a directinteraction with other granule components. |
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