Uptake of Adenosine by Isolated Rat Brain Capillaries |
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Authors: | P H Wu J W Phillis |
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Institution: | Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada |
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Abstract: | Abstract: Adenosine uptake by isolated rat brain capillaries is a carrier-mediated, temperature- and pH-sensitive process. The K m value for adenosine uptake is 4.74 μ m and the V max is 21.7 picomol/mg protein/10 min. This is a high-affinity uptake system that can be cross-inhibited by several nucleosides and by the adenosine analogs tubercidin and 5'-deoxyadenosine. The uptake is very sensitive to inhibition by papaverine, hexobendine, and dipyridamole. These results confirm the existence of a nucleoside transport system associated with the blood-brain barrier observed during in vivo studies. |
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Keywords: | Adenosine transport Capillaries Rat brain |
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