Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch |
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Authors: | Nicole Brassard Line Brissette Daniel Lord Sylvie Laliberté |
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Affiliation: | (1) Département des Sciences Biologiques, Gref, Université du Québec À Montréal, Succ. Centre-Ville, C.P. 8888, H3C 3P8 Montréal, Québec, Canada;(2) Département des Sciences Fondamentales, Université du Québec á Chicoutimi, 555 Boulevard de l'Université, G7H 2B1 Chicoutimi, Québec, Canada |
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Abstract: | Factors were defined for elongation, rooting and acclimatization of micropropagated shoots ofLarix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 M 6-benzyladenine (BA) and 1 M indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 M BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 M of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth. |
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Keywords: | acclimatization conifer Larix x eurolepis mature material plagiotropism rooting stem elongation |
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