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High-resolution mapping of the barley Ryd3 locus controlling tolerance to BYDV |
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| Authors: | Thomas Lüpken Nils Stein Dragan Perovic Antje Habekuß Albrecht Serfling Ilona Krämer Urs Hähnel Burkhard Steuernagel Uwe Scholz Ruvini Ariyadasa Mihaela Martis Klaus Mayer Rients E Niks Nicholas C Collins Wolfgang Friedt Frank Ordon |
| Institution: | 1. Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany 2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Stadt Seeland (OT) Gatersleben, Germany 3. Munich Information Center for Protein Sequences/Institute of Bioinformatics and Systems Biology (MIPS/IBIS), Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, 85764, Neuherberg, Germany 4. Department of Plant Breeding, Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 386, 6700, Wageningen, The Netherlands 5. Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia 6. Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany |
| Abstract: | Barley yellow dwarf disease (BYD) is transmitted by aphids and is caused by different strains of Barley yellow dwarf virus (BYDV) and Cereal yellow dwarf virus (CYDV). Economically it is one of the most important diseases of cereals worldwide. Besides chemical control of the vector, growing of tolerant/resistant cultivars is an effective way of protecting crops against BYD. The Ryd3 gene in barley (Hordeum vulgare L.) confers tolerance to BYDV-PAV and BYDV-MAV and the locus was previously mapped on the short arm of barley chromosome 6H near the centromere. We applied a strategy for high-resolution mapping and marker saturation at the Ryd3 locus by exploiting recent genomic tools available in barley. In a population of 3,210 F2 plants, 14 tightly linked markers were identified, including 10 that co-segregated with Ryd3. The centromeric region where Ryd3 is located suffers suppressed recombination or reduced recombination rate, suggesting potential problems in achieving (1) map-based cloning of Ryd3 and (2) marker selection of the resistance in breeding programmes without the introduction of undesirable traits via linkage drag. |
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