A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield |
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Authors: | M Furutani M Iwaki T Yagi M Iida K Horiike M Nozaki |
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Affiliation: | Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Japan. |
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Abstract: | 1. Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively. |
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