变性梯度凝胶电泳法研究断奶仔猪粪样细菌区系变化

利用PCR和DGGE技术分析了12头仔猪在断奶后其粪样细菌区系的变化。粪样细菌16S rDNA的V6～V8可变区经PCR扩增，扩增产物经DGGE电泳后再进行相似性分析。结果表明，仔猪断奶当天粪样 DGGE谱带少，同窝仔猪间图谱相似。断奶后，随着断奶时间的推移，每头仔猪的DGGE图谱带逐渐增多，变得复杂和多样，仔猪个体间DGGE图谱差异逐渐增大。仔猪是否同窝以及所采食日粮类型对DGGE图谱没有明显影响。相似性分析还表明，日粮中添加寡果糖的仔猪在断奶后第1周，其粪样微生物区系变化迅速，而后缓慢。

Development of Bacterial Community in Faeces of Weaning Piglets as Revealed by Denaturing Gradient Gel Electrophoresis

PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the deve- lopment of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated and diverse. Each individual piglet had its unique DGGE profile, with low similarity between piglets. The source of sow and the diet showed no appa- rent effect on the DGGE profiles. For the piglets fed a diet containing oliogofructose, DGGE similarity analysis showed bacterial community in the faeces developed fast during the first week and relatively slow later after weaning.