| Abstract: | Prior to completion of aggregation and the beginning of multicellular differentiation, the amoebae of Dictyostelium discoideum assume two distinct phases with characteristic changes in cellular movement, shape and adhesiveness. These two phases of amoeboid behaviour have been studied with respect to the quantitative analysis of the intracellular adenosine phosphates, using both enzymatic and chromatographic techniques. A higher intracellular ATP level and energy-charge has been found for the actively moving, non-adhesive amoebae as compared to the flattened, mutually adhesive cells. The importance and possible role of ATP in regulating amoeboid form, movement and cell adhesion is discussed. |
