Genetic composition of the recombinant congenic strains |
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Authors: | A P M Stassen P C Groot J T Eppig P Demant |
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Institution: | (1) Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands, NL;(2) Mouse Genome Informatics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA, US |
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Abstract: | For the study of biological phenomena influenced by multiple genes in mice, the Recombinant Congenic Strains (RCS) have been
developed. An RCS series comprises approximately 20 homozygous strains, each of which contains on average 87.5% genes of a
common background strain and 12.5% of a common donor strain. In an RCS series, non-linked genes involved in the control of
a multigenic trait become distributed into different recombinant congenic strains. In this way a multigenic trait is transformed
into a series of single gene traits in which each gene can be studied individually. For the ability to use the strength of
the recombinant congenic strains system to its full extent, a thorough genetic characterization is indispensable. We have
typed the CcS/Dem and OcB/Dem series for 611 and 550 markers, respectively. This results in a genetic characterization sufficient
to detect most donor strain genes. In addition, we report the genetic characterization of the HcB/Dem and HcB(N4)/Dem series.
Strains of the latter series contain on average 6.25% of the donor strain genome. Both series have been typed for 130 markers.
All the typing data have been deposited in the Mouse Genome Database at The Jackson Laboratory.
Received: 11 July 1995 / Accepted: 18 August 1995 |
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