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Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica
Authors:Alicia S Linford   Heriberto Moreno   Katelyn R Good   Hanbang Zhang   Upinder Singh  William A Petri
Affiliation:(1) Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA;(2) Department of Medicine, University of Virginia, Charlottesville, Virginia, USA;(3) Department of Internal Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California, USA;(4) Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA;(5) Department of Pathology, University of Virginia, Charlottesville, Virginia, USA
Abstract:

Background  

Entamoeba histolytica is an intestinal protozoan parasite of humans. The genome has been sequenced, but the study of individual gene products has been hampered by the lack of the ability to generate gene knockouts. We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica.
Keywords:
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