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| 荧光标记DNA扩增片段长度多态性方法的改进 | |
| 引用本文： | 杨凯,宋婉,张春庆,贾继增. 荧光标记DNA扩增片段长度多态性方法的改进[J]. 生物化学与生物物理进展, 2001, 28(2): 256-258 |
| 作者姓名： | 杨凯 宋婉 张春庆 贾继增 |
| 作者单位： | 1. 中国农业科学院作物品种资源研究所，北京 100081 2. 北京林业大学生物学院，北京 100083 3. 山东农业大学农学系，济南 200000 |
| 摘 要： | 采用常规PCR试剂和合成的接头和引物，其中MseⅠ引物为荧光标记物FAM标记，并对扩增片段长度多态性(AFLP)程序进行了改进，优化了PCR反应和电泳条件，建立了一个新的、有效的反应体系，降低了实验费用，经比较实验结果与AFLP荧光标记试剂盒的实验效果一致. |
| 关 键 词： | 自动测序，荧光标记引物，扩增片段长度多态性(AFLP) |
| 收稿时间： | 2000-04-24 |
| 修稿时间： | 2000-06-07 |
An Improved Method of Fluorescent Labeled Amplified Fragment Length Ploymorphism |
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| YANG Kai,SONG Wan,ZHANG Chun-Qing and JIA Ji-Zeng. An Improved Method of Fluorescent Labeled Amplified Fragment Length Ploymorphism[J]. Progress In Biochemistry and Biophysics, 2001, 28(2): 256-258 | |
| Authors: | YANG Kai SONG Wan ZHANG Chun-Qing JIA Ji-Zeng |
| Affiliation: | Chinese Academy of Agricultural Sciences, Institute Crop of Germplasm Research(ICGR), Beijing 100081, China;Coolege of Biological Science, Beijing Frost University, Beijing 100083, China;Deparment of Agricnltrral Science, Shandong Agricultural University, Jinan 200000, China;Chinese Academy of Agricultural Sciences, Institute Crop of Germplasm Research(ICGR), Beijing 100081, China |
| Abstract: | A new and efficient reaction system has been set up, in which the MseⅠ primers were fluorescent labeled for auto-sequencer. PCR reagents and primers and adapters of MseⅠ and EcoRⅠ, which were synthesized, the AFLP protocol has been modified, and reaction and electrophoresis conditions were optimized, the results obtained can be comparable to that of AFLP fluorescent labeled AFLP kits with less cost. |
| Keywords: | auto-sequencer fluorescent labeled amplified fragment polymorphism(AFLP) |
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